Beer’s Law Plots
How is a law molar absorptivity of beer frame?
Beers law establishing the molar absorptivity times path length cells times the concentration of the solution is equal to the absorbance. The equation is: A = ebc. where e = molar absorptivity, b = cell path length in cm, c = concentration in moles / liter. A is measured from the spectrum, usually a UV / visible spectrum, and has no units. b is usually indicated by the manufacturer of cells, and the concentration is determined by an analytical technique. A plot Beers law requires you to plot A of C as a function of various concentrations. The line will linear. The slope of the line gives the molar absorptivity (in reality, and b) since the cell path length is known, and is usually 1.00 cm. Remember, slope is rise / run I hope this answers your question.
Fall 2007 Student Chemistry Video – Beer’s Law Plot