Beer’s Law Formula
Beer's law – Please help me understand! 10 points?
In particular, we will prepare a graph of absorbance versus [Co2 +] for following solutions – Prepare by diluting the specific volume of 0.2500 M stock solution of Co2 + 25.00 ml each. The absorbance of each solution was taken at 394 nm. Volume mL0.000 Absorbance 0.00 2.50 5.00 7.50 10.00 mL0.165 mL0.320 mL0.485 mL0.721 mL0.610 12.00 (a) Find the slope I can not find the slope! For my graphic excellence, needed to find a formula to determine the concentration of CO2 [+] and I a beer from the law = EBC. "I can rearrange C = A / eb. Then I used any value of absorption was there. Dividing by 394. And now, my slope is 394! (That's right) In response, the slope is 6.15 L / mol. Please help me understand the answer!
you do not get + the CO2 concentration of the formula to use the dilution formula M1 =. 25; volumes vary, M2 want; V2 = 25 for 2.5 ml, for example, M = 0.25 * 2.5 / 25 = 0.025 moles per liter that the rest are 0.025, 0.05, 0.075, 0.1, 0.12 moles per liter , they are recorded in the absorbance values and Excel gives the slope as 6.1 is a calibration curve
Management Cybernetics: The Law of Requisite Variety