Beer Lambert Law Absorbance
Using the Lambert-Beer's law to find the molar absorptivity.?
A solution known to contain 0.026 molarity Cu2 + complexes with ethylenediamine that produces an absorbance of 0.37 in a 1.0 cm cell path length at a selected wavelength. Calculate E (molar absorptivity) for these conditions.
* A = b * c epsilon A is measured absorbance (0.37M) epsilon is the wavelength of molar absorptivity (? M ^ -1 cm ^ -1) b = path length (1.0cm) c = concentration (0.026M) A bc = epsilon / epsilon = 14.2
Absorbance Spectrophotometry